WebJan 16, 2024 · BPyV-2 DNA was detected in 3/10 (30%) ground beef samples, whereas BPyV-3 was not detected in the samples. Application of RCA did not increase the detection rate in ground beef samples. Sequence analysis of the PCR products indicated the presence of BPyV-1, BPyV-2a and BPyV-2b. The whole genome of a BPyV-1 strain from ground … WebThe early region of bovine polyomavirus (BPyV) was tested for its cell transformation potential employing an assay of dense focus formation. Dense foci of morphologically transformed cells were observed upon transfection of primary rodent cells with a plasmid construct encoding the complete early region of BPyV under the transcriptional control of …
きゅうり 黄化病 : こうち農業ネット
きゅうり 一般名称 黄化病 学術名称 Beet pseudoyellows virus (BPYV) (Cucumber yellows virus(CYV)) 症状 収穫開始前後に発生する。 葉脈間に黄緑色の小斑点が多数発生し、後に黄化する。 激しく発病すると、側枝の発生や伸びが悪くなり、曲がった果実が多くなる。 発生条件 ほ場周辺の雑草類が伝染源となり、ここからオンシツコナジラミによってきゅうりに伝染すると考えられている。 接触伝染、種子伝染、土壌伝染はしない。 対策 (1)媒介昆虫であるオンシツコナジラミを防除する。 (2)ほ場周辺の除草を徹底する。 形態情報 防除指針 ツイート Web露地キュウリの黄化病の初期病徴は、葉表が淡く黄化し、葉裏にツヤがなくなる。 また、葉脈間を太陽光に透かす等の透過光で肉眼観察すると1mm程度の無数の小点(退色小 … burt whitmire funeral home fort payne al
Bovine polyomavirus, a cell-transforming virus with tumorigenic ...
WebBeet pseudo-yellows virus (BPYV) (Crinivirus, Closteroviridae) BPYV is a crinivirus transmitted by whitefly in the mode semi-persistent . It is mostly found in sheltered crops where it causes like symptoms yellowing. WebWe have established a reliable and sensitive PCR assay which can detect 10 pg BPyV DNA. This was developed using a BPyV infected cell line (a gift from D. Westcott, CVL) as a source of positive control DNA, and our own stock of MDBK cells as a source of negative control DNA. This assay can be used as a routine screen for the detection of BPyV ... WebAug 3, 2024 · BPYV is endemic in cucurbit crops in Greece for more than 20 years especially in Northern Greece where Trialeurodes vaporariorum West., the vector of BPYV, is highly abundant (Orfanidou et al. 2024). However, this is the first time we detected the virus in lettuce crops. burt whitt