Hcs lipidtox red neutral lipid stain
WebDec 2, 2024 · Adipocyte lipid droplet staining was performed with HCS LipidTOX™ Deep Red Neutral Lipid Stain (Thermo Fisher, Waltham, MA, USA). After washing cells with … WebHCS LipidTOX Deep Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX …
Hcs lipidtox red neutral lipid stain
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WebSep 5, 2016 · Lipid droplets (LDs) are ubiquitous, dynamic organelles and function as a storage depot for neutral lipids, including triglycerides and cholesterol esters (Walther … WebThe LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, … HCS LipidTOX™ Red neutral lipid stain was developed to characterize the potentially … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes
Web4 rows · HCS LipidTOX Deep Red neutral lipid stain was developed to characterize the potentially toxic ... WebSep 24, 2024 · After washing in DPBS, the neutral lipid content in the cells and the bacteria was revealed by at least 30 min incubation with a solution of HCS LipidTOX Red Neutral Lipid Stain (LipidTOX Red, ThermoFisher Scientific) diluted 1/300. Plates were then read using the high-content screening (HCS) technology. Fluorescence Microscopy
WebDec 14, 2009 · Neutral lipid droplets and phospholipidosis were analyzed using Invitrogen's HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit for image-based high-content screening assays. Staining … WebJul 11, 2014 · Immunofluorescence and HCS LipidTox Red neutral lipid staining. Cells grown on coverslip or in labtec II chamber slides (Nunc) were fixed in 4% paraformaldehyde for 1 h at room temperature, washed with PBS, and permeabilized in 0.1% Triton X-100 for 5 min or 0.5% saponin for 5 min. After incubation in blocking solution (5% BSA, 1% fish …
WebJun 17, 2024 · In addition, 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, A-62248; 1:500) and HCS LipidTOX Deep Red Neutral Lipid Stain (Thermo Fisher Scientific, H34477; 1:500) were added to the …
WebSep 11, 2013 · For neutral lipid staining, lipid droplets were incubated for 10 min in HCS LipidTOX Neutral Red Lipid Stain (Molecular Probes) as recommended by the supplier. A Zeiss Axio Imager microscope with fluorescence and Nomarski optics and a Roper CoolSnap HQ2 camera coupled to a Zeiss AxioVision driver was used. spider factsyyyyWebLipidTox stains live cells nicely, and the lipid droplets are stable for a few days as long as the cells are healthy. There will be basal uptake of the stain into non-lipid droplet containing ... spider fact fileWebNov 9, 2024 · Staining of DMBM-2, J774, and RAW264.7 macrophages with HCS LipidTOX Deep Red neutral lipid stain and HCS LipidTOX Green phospholipidosis detection reagent after exposure to solvent (control), 7.5 µM amiodarone (Amio), and 7.5 µM chloroquine (Chloro). Nuclei are stained in blue. Scale bar 100 µm. Figure 3. spider factsyyyWebDec 2, 2024 · Adipocyte lipid droplet staining was performed with HCS LipidTOX™ Deep Red Neutral Lipid Stain (Thermo Fisher, Waltham, MA, USA). After washing cells with PBS three times, the adipocytes were fixed with 4% formaldehyde and incubated for 30 min at room temperature. spider falls in bathtubWebCatalog number: H34475. The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS … spider facts for kids printableWebWhile Nile Red staining has been considered unreliable for identifying lipid structures in live C. elegans, our work demonstrates that highly reliable results of lipid structure imaging can be obtained from vital Nile Red staining at the concentration we employed when coupled with 2p-FLIM excitation at 920 nm and phasor analysis. In our work ... spider fangs close upWebOct 30, 2024 · Prepare the counterstain/fixation solution by adding 3 mL of 16% formaldehyde and 6 μL of Hoechst 33342 (Component C) to 9 mL PBS. 1.4 Method This protocol is developed for HepG2 cells and has also … spider fashions private limited