2024 Klf5 chip

Klf5 chip

Author: giol

August undefined, 2024

WebIndeed, Klf5-null mice show developmental defects at blastocyst stage, due to the defective establishment of the inner cell mass. Moreover, Klf5 knockdown in ESCs results in the … WebFeb 21, 2024 · Krüppel-like Factor 5 (KLF5) is a cell-intrinsic oncogene to promote tumorigenesis. However, the cell-extrinsic effects of KLF5 on suppressing the immune …

TP63, SOX2, and KLF5 Establish a Core Regulatory Circuitry That ...

WebJan 24, 2024 · ChIP assays were conducted to verify the enrichment of the potential binding sites by KLF5 antibody in BT-549 cells. The ChIP results were analyzed by qPCR and nucleic acid electrophoresis. The dual-luciferase reporter assays were performed to validate the interaction between KLF5 and the first binding site (E1). WebKLF5 ChIP-seq on human GM12878 Nucleic acid type DNA Size range 450-650 Platform Illumina HiSeq 2000 Controls ENCSR398JTO Attribution Lab Michael Snyder, Stanford Award U54HG006996 (Michael Snyder, Stanford) Project ENCODE External resources GEO:GSE127670 References PMID:31969180 PMCID:PMC7333332 PMCID:PMC6724667 … python to_csv 改行コード lf

TP63, SOX2, and KLF5 Establish a Core Regulatory Circuitry That ...

WebOct 15, 2016 · We show that Klf5 is also an essential mediator of skeletal muscle regeneration and myogenic differentiation. During muscle regeneration after injury (cardiotoxin injection), Klf5 was induced in the nuclei of differentiating myoblasts and newly formed myofibers expressing myogenin in vivo. WebOct 1, 2024 · TP63, SOX2, and KLF5 Establish a Core Regulatory Circuitry That Controls Epigenetic and Transcription Patterns in Esophageal Squamous Cell Carcinoma Cell Lines - ScienceDirect Gastroenterology Volume 159, Issue 4, October 2024, Pages 1311-1327.e19 Original Research Full Report: Basic and Translational—Alimentary Tract WebOct 7, 2024 · To determine direct gene targets of KLF5, we defined the cistrome of KLF5 using ChIP-seq in both Calu-3 and 16HBE14o- lung epithelial cells lines. Occupancy site concordance analysis revealed that KLF5 co-localized with the active histone modification H3K27ac and also with binding sites for CCAAT Enhancer Binding Protein Beta (C/EBPβ). python to_deterministic

KLF5 Gene - GeneCards KLF5 Protein KLF5 Antibody

Network analysis of KLF5 targets showing the potential oncogenic role …

WebWe performed RNA sequencing, immunohistochemistry, western blotting, real time-PCR, ELISA, luciferase assay, chromatin immunoprecipitation (ChIP), and flow cytometry to demonstrate the effects of KLF5 on CD8 + T cell infiltration and related molecular mechanism. Single-cell RNA sequencing and spatial transcriptomics analysis were … WebNational Center for Biotechnology Information python to_excel sheet_nameWebOct 1, 2024 · The expression of KLF5 was initially examined, it was top ranked in ESCC samples relative to other types of cancers in either TCGA 38 or Cancer Cell Line … python to_excel sheet

"WebOct 22, 2009 · The Krüppel-like factor 5 (KLF5) is a zinc-finger transcription factor promoting cell proliferation, cell-cycle progression and survival. A high expression level of KLF5 mRNA has been shown to be associated with shorter breast cancer patient survival. However, the mechanism of KLF5 action in breast … " - Klf5 chip

Klf5 chip

Klf5 regulates muscle differentiation by directly targeting ... - eLife

WebHIF-1α活化对肾小管间质炎症和纤维化的影响及其机制探讨.pdf

Did you know?

WebTo investigate whether KLF5 acetylation affects the binding of KLF5 to KBE and TIE of MYC promoter, we conducted a ChIP assay in HepG2 cells transfected with pcDNA3-FLAG … WebSep 7, 2024 · Overall, five samples of KLF5 ChIP-Seq datasets were obtained, plus PCGs and lncRNAs with a KLF5 binding site in at least two samples were selected for the first step. Second, the differently expressed lncRNAs and PCGs were obtained from the intersection results of the RNA-Seq dataset (GSE50760) and Cancer RNA-Seq Nexus database. ...

WebApr 13, 2024 · ChIP-seq of B2B1 samples was successful when immunoprecipitating EGFR chimeras, ... NEAT1) and transcription factors of the AP1 (JUNB) and KLF (KLF5) families. WebAntibodies that detect KLF5 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ChIP and ELISA. These antibodies …

WebMar 21, 2024 · KLF5 (KLF Transcription Factor 5) is a Protein Coding gene. Diseases associated with KLF5 include Cardiomyopathy, Dilated, 1Ff and Sigmoid Disease.Among its related pathways are White fat cell differentiation and RNA Polymerase I Promoter Opening.Gene Ontology (GO) annotations related to this gene include DNA-binding … WebNov 9, 2024 · Although Klf5 has the ability to induce ICM and TE genes based on our ChIP-seq data, Klf5 mediates gene transcription in a cell-type- and context-specific manner. In pluripotent ESCs, Klf5 overexpression induced multiple pluripotency transcription factors, including Nanog, ...

WebAntibodies that detect KLF5 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ChIP and ELISA. These antibodies target KLF5 in Human, Mouse and Rat samples. Our KLF5 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit …

WebOct 15, 2024 · Chromatin immunoprecipitation (ChIP) assays indicate that the Fgf4 locus is occupied by Klf5, suggesting direct regulation of Fgf4 by Klf5. In terms of the emergence of Nanog + pluripotent EPI cells, the phenotypes of Klf5 KO embryos can be reversed by either Fgfr or ERK inhibition. python to_csv 中文乱码http://genesdev.cshlp.org/content/36/13-14/822.long python to_excelWebFeb 20, 2024 · ChIP-seq data Rogerson et al., 2024 demonstrate that KLF5 strongly binds to the JUP and CCNE1 enhancers but low levels are observed at the MYBL2 enhancer . KLF5 depletion ( Figure 3—figure supplement 1H ) caused reduced expression of all three target genes ( Figure 3H ). python to_csv errorWebIn epigenetic and transcriptional analyses of ESCC cell lines, we found the transcription factors TP63, SOX2, and KLF5 to be part of a core regulatory network that determines chromatin accessibility, epigenetic modifications, and gene expression patterns in these cells. A combination of epigenetic i … python to_denseWebAug 31, 2024 · Klf5 regulates muscle differentiation via directly targeted muscle-specific genes in cooperation with MyoD in mice. Organism. Mus musculus. Experiment type. … python to_excel indexWebJun 11, 2024 · For instance, ChIP-sequencing showed that KLF5 binding sites in C2C12 myotubes and 3T3-L1 adipocytes differ, and only a small fraction of sites are common between the two cell types (Figure 4A). Moreover, even in the same C2C12 cells, differentiation alters KLF5 bindings sites (Figure 4B) . Likewise, KLFs may control … python to_excel函数WebFeb 10, 2008 · Interestingly, the ChIP-on-chip data revealed a weak signal of Klf2, Klf4 and Klf5 occupancies at the Fgf5 and Nes promoters. This binding was confirmed using ChIP–qPCR assays, and showed... python to_excel 不覆盖